These clubs are participating in National League club divisions in the 2025-26 season.
Lists are subject to change. Additional waitlisted clubs may be added.
Premier 1
(formerly Elite 64)
| Premier 1 – Frontier | |
| Atletico Dallas Youth | TX-N |
| BVB International Academy Texas | TX-N |
| D’Feeters Kicks Soccer Club | TX-N |
| Dallas Surf Soccer Club | TX-N |
| HTX Soccer Club | TX-S |
| Houston Surf Soccer Club | TX-S |
| Solar Soccer Club | TX-N |
| Premier 1 – Great Lakes | |
| Club Ohio | OH |
| FC Dayton | OH |
| Fort Wayne United Futbol Club | IN |
| Indy Eleven Academy | IN |
| NWI Lions United | IN |
| Total Futbol Academy | OH |
| Premier 1 – Pacific | |
| AYSO United So Cal | CA-S |
| CDA Slammers | CA-S |
| Empire Surf | CA-S |
| Excel Soccer Academy | AZ |
| FC Premier | CA-S |
| Legends FC | CA-S |
| Legends FC San Diego | CA-S |
| Legends FC SD North | CA-S |
| Los Angeles Soccer Club | CA-S |
| Phoenix Rising FC | AZ |
| Rebels San Diego Soccer Club | CA-S |
| Sporting California | CA-S |
| Strikers FC | CA-S |
| Premier 1 – Piedmont | |
| Alliance Soccer Club | GA |
| Atlanta Fire United Soccer Association | GA |
| Bulls Rush FC | SC |
| Carolina Elite Soccer Academy | SC |
| Charlotte Soccer Academy | NC |
| United FC-Furman | SC |
Girls – Premier 2
(formerly Elite 64 Regional League)
| Premier 2 – Desert | |
| Arizona Arsenal Soccer Club | AZ |
| Arizona Rush | AZ |
| AYSO United Arizona | AZ |
| CCV Stars | AZ |
| FC Tucson Youth | AZ |
| RSL-AZ West Valley | AZ |
| RSL-AZ Southern | AZ |
| Premier 2 – Great Lakes | |
| Cincinnati United Premier | OH |
| Cincinnati West Soccer Club | OH |
| Elite FC | OH |
| Fairfield Optimist Soccer Club | OH |
| Hoosier Futbol Club | IN |
| Indy Eleven Academy | IN |
| Indy Premier SC | IN |
| Kings Hammer – NKY Academy | KY |
| Lexington Sporting Club | KY |
| Union FC Indy | IN |
| Premier 2 – Lake Shores | |
| Libertyville FC 1974 | IL |
| MI Jaguars FC | MI |
| Michigan Futbol Academy | MI |
| Michigan Tigers | MI |
| Team Chicago | IL |
| Premier 2 – North | |
| Chicago Inferno | IL |
| Croatian Eagles Soccer Club | WI |
| Evolution Soccer Club | IL |
| FC Stars Soccer Club | IL |
| Raptors / Crystal Lake Force | IL |
| WIUFC | WI |
| Premier 2 – Pacific | |
| CDA Slammers | CA-S |
| Crusaders Soccer Club | CA-S |
| FC Man United | CA-S |
| Legends FC – San Diego | CA-S |
| Legends FC SD North | CA-S |
| Soccer Club of Oceanside | CA-S |
| Southwest Sporting | CA-S |
BOYS – Premier 1
(formerly Elite 64)
| Premier 1 – FRONTIER | |
| Atletico Dallas Youth | TX-N |
| Dallas Surf SC | TX-N |
| D’Feeters Kicks SC | TX-N |
| HTX SC | TX-S |
| Houston Surf | TX-S |
| Solar SC | TX-N |
| Sting SC | TX-N |
| Premier 1 – Great Lakes | |
| Century United of Pittsburgh | PA-W |
| Fort Wayne United Futbol Club | IN |
| Hoosier Futbol Club | IN |
| Indy Premier SC | IN |
| Pittsburgh Independence | PA-W |
| Spire FC | OH |
| Premier 1 – Midwest | |
| Central Illinois United | IL |
| FC 1974 Libertyville | IL |
| Madison 56ers | WI |
| Michigan Rangers FC | MI |
| Midwest United SCOR | MI |
| PFC – Evo | IL |
| TKO Premier SC | MI |
| Premier 1 – Northeast | |
| Cedar Stars Academy – Hudson Valley | NY-E |
| Cedar Stars Academy – North | NJ |
| French Football Academy | NY-E |
| Future Soccer Academy | NJ |
| PDA SC Vistula | NJ |
| Players Development Academy Hibernian | NJ |
| PSC Academy | NJ |
| Premier 1 – Pacific | |
| Empire Surf | CA-S |
| Excel Soccer Academy | AZ |
| FC Deportivo AZ | AZ |
| Legends FC | CA-S |
| Legends FC San Diego | CA-S |
| Legends FC SD North | CA-S |
| Los Angeles Soccer Club | CA-S |
| Nomads Soccer Club | CA-S |
| Phoenix Rising FC | AZ |
| Playmaker Futbol Academy | AZ |
| Rebels San Diego Soccer Club | CA-S |
| Soccer Club of Oceanside Breakers | CA-S |
| Southwest SC Sporting USA | CA-S |
| Strikers FC | CA-S |
| Premier 1 – SOUTHEAST | |
| GGS – Brave SC | FL |
| Juventus Academy Miami | FL |
| Miami Breakers FC | FL |
| One FC | FL |
| Pinecrest Premier Soccer Club | FL |
| SABR Team Boca | FL |
| Strikers Miami FC | FL |
| West Pines United FC | FL |
BOYS – Premier 2
(formerly Elite 64 Regional League)
| Premier 2 – Chicagoland | |
| Barça Academy Chicago | IL |
| Chicago City Soccer Club | IL |
| Chicago Kics Football Club | IL |
| Evolution Soccer Club | IL |
| FC Stars Soccer Club | IL |
| Galaxy Soccer Club | IL |
| Millennium Soccer Association | IN |
| NWI Lions United | IN |
| Premier 2 – Desert | |
| Arizona Arsenal Soccer Club | AZ |
| Arizona Soccer Club – Thunder | AZ |
| Brazas Futebol Club | AZ |
| CCV Stars | AZ |
| FC Tucson Youth | AZ |
| North Scottsdale SC Sandsharks | AZ |
| Paris Saint-Germain Academy Phoenix | AZ |
| Phoenix Rising FC SE Valley | AZ |
| Phoenix Rising FC West Valley | AZ |
| RSL- AZ Southern | AZ |
| RSL-AZ West Valley | AZ |
| Spartans FC Academy | AZ |
| Premier 2 – ERIE SHORES | |
| Cincinnati United SC | OH |
| Cleveland Football Academy | OH |
| Club Ohio | OH |
| Elite FC | OH |
| FC Dayton | OH |
| Toledo Celtics SC | OH |
| Premier 2 – Gateway | |
| KC Fusion Soccer Club | KS |
| OP Soccer Club | KS |
| St. Louis Scott Gallagher | MO |
| Toca FC | KS |
| Premier 2 – North | |
| Chicago Inferno | IL |
| Croatian Eagles Soccer Club | WI |
| Elmbrook United | WI |
| FC Wisconsin | WI |
| FC1 Academy | IL |
| Libertyville FC 1974 | IL |
| Madison 56ers | WI |
| North Shore United Soccer Club | WI |
| Rockford Raptors FC | IL |
| WIUFC | WI |
| Premier 2 – Ohio Valley | |
| Columbus Express | IN |
| Indy Premier SC | IN |
| Kings Hammer – NKY Academy | KY |
| Total Futbol Academy | OH |
| Union FC Indy | IN |
| USA of Indiana | IN |
| Premier 2 – Pacific | |
| AYSO United CA | CA-S |
| Crusaders SC | CA-S |
| FC Man United | CA-S |
| Legends FC San Diego | CA-S |
| Legends FC SD North | CA-S |
| SoCal Elite FC | CA-S |
| Premier 2 – Piedmont | |
| Bulls Soccer Club | SC |
| Carolina Elite Soccer Academy | SC |
| Charleston Soccer Club | SC |
| Coast Futbol Alliance | SC |
| South Carolina United FC | SC |
| United FC-Furman | SC |
| Premier 2 – SUNSHINE | |
| Doral SC | FL |
| Key Biscayne SC | FL |
| PSG Academy FL | FL |
| PSL Soccer Club Hurricanes, Inc. | FL |
| Strikers Miami FC | FL |
| Tropical SC | FL |
| W&H America | FL |
| West Pines United FC | FL |